You wanted to study the assembly of preinitiation complex in eukaryotes. This is by the means of protein-protein interaction. Which of these methods you can’t absolutely put in use there?
a) Footprinting
b) Crystal structure
c) EMSA
d) Primer extension
Q.3.
For performing EMSA what type of gel should be used?
a) Native
b) Denaturing SDS-PAGE
c) Non-denaturing SDS PAGE
d) Zymography
Q.4.
While performing EMSA, in which situation will you see the band highest?
a) You add TFIID, TFIIA and polymerase
b) You add TFIID, TFIIB, TFIIF and polymerase
c) You add TFIID, TFIIA, TFIIF and polymerase
d) You add TFIIA, TFIIB, TFIIF and polymerase
Q.5.
Which of these is not true for TFIIH?
a) It is a helicase
b) On initiation the RNA pol starts transcribing leaving TFIIH at pre-IC
c) It has kinase activity
d) It is needed in Double Strand Break (DSB) repair
Q.6.
A mediator helps to connect ___________
a) Enhancer to DPE
b) Silencer to Enhancer
c) Enhancer to TAF
d) Insulator to silencer
Q.7.
Which factor stimulated phosphorylation of CTD of RNA polymerase II?
a) TFIIA
b) TFIIF
c) TFIIH
d) TFIIE
Q.8.
The absence of which factor will prevent TFIIH and TFIIE recruitment?
a) F
b) D
c) B
d) A
Q.9.
Which of this is untrue about TBP?
a) It is a part of TFIID
b) It makes a kink in the DNA
c) It is orientation independent
d) It is sequence independent
Q.10.
While in vitro transcription your RNA pol is stalled at a position due to mistreatment. Which factor could you act to help the RNA pol overcome the stalled stage?
a) TFIID
b) TFIIF
c) TFIIS
d) TFIIH
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