Secretion of overexpressed proteins by fusion to a secretion signal can be useful because it helps in bringing about correct _______ and _____ protein folding.
a) glycosylation, enhance
b) glycosylation, not affect
c) phosphorylation, enhance
d) phosphorylation, not affect
Q.2.
The enzyme used for breaking down of sucrose into glucose and fructose is called as _______________
a) sucrase
b) glucanase
c) maltase
d) invertase
Q.3.
For introduction of exogenous DNA into S.cerevisiae ______ is added along with polyethylene glycol and heat shock treatment.
a) calcium chloride
b) sodium acetate
c) lithium acetate
d) magnesium chloride
Q.4.
Protoplast transformation is less time consuming than lithium acetate for transformation.
a) True
b) False
Q.5.
Schizosaccharomyces pombe is yeast and it reproduces by __________
a) budding
b) fissing
c) either budding or fissing
d) a method of reproduction other than budding or fissing
Q.6.
The leumarker encodes which enzyme?
a) orotidine-5’-phosphate decarboxylase
b) beta isopropylmalate dehydrogenase
c) orotidine-5’-phosphate decarboxylase or beta isopropylmalate dehydrogenase
The replacement of one plasmid by another by introduction of the second plasmid into strain containing the first, followed by removal of the first by counterselection is known as __________
a) shuffle vector
b) plasmid shuffle
c) counterselection plasmid
d) combination plasmid
Q.8.
Promoters can be divided basically into how many types?
a) 1
b) 2
c) 3
d) 4
Q.9.
nmtgene is used for _____ biosynthesis.
a) uracil
b) adenine
c) cytosine
d) thiamine
Q.10.
The promoter from fbpgene is suppressed in the presence of glucose.
a) True
b) False
Q.11.
Species which can grow on _______ as a carbon source are called as methylotropic.
a) methylase
b) methanol
c) methane
d) methanoic acid
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