T protein is required for replication. It is a product of ____________
a) delayed early transcription
b) late transcription
c) early transcription
d) both early and late replication
Q.2.
Vaccinia virus is a commonly used virus and it is a member of pox virus family. It has a large genome of _____________ and can accept at least ____________ of foreign DNA.
a) 200kb, 25kb
b) 1000kb, 200kb
c) 500kb, 50kb
d) 800kb, 100kb
Q.3.
Vaccinia virus produces its own ______ which is functional in _____ of infected cells where the virus also replicates.
a) DNA polymerase, nucleus
b) DNA polymerase, cytoplasm
c) RNA polymerase, cytoplasm
d) RNA polymerase, nucleus
Q.4.
It is beneficial to use retrovirus for genetic manipulation of cells in the culture.
a) True
b) False
Q.5.
LNSX is a retrovirus and is combined with ______ in order to create a shuttle vector.
a) eukaryotic plasmid
b) prokaryotic plasmid
c) either eukaryotic or prokaryotic plasmid
d) yeast plasmid
Q.6.
The retroviral transfected DNA is infected into mammalian cells by helper retrovirus. Helper retrovirus should not be packaged and the method adopted for this is?
a) Deletion of the packaging signal in the helper genome
b) Deletion of multiple parts of the helper genome
c) Division of the helper genome into several pieces
d) All of the mentioned
Q.7.
Choose the incorrect statement in respect to MACs.
a) They are mammalian or human artificial chromosomes
b) Large stretches of DNA (in excess of 100 kb) can be inserted
c) A selectable marker is required
d) It can be maintained for long term
Q.8.
Centromere is the most important component of the human artificial chromosome. It is constructed from tandemly repeated copies ofbp of ______ DNA sequence.
a) alpha satellite
b) beta satellite
c) satellite 2
d) satellite 1
Q.9.
Constitutive promoters are majorly obtained from ________
a) fungi
b) bacteria
c) mammalian cells
d) viral cells
Q.10.
In naturally occurring bacterial systems, tetracycline binding repressor protein binds in the _____ of tetracycline to the control sequence and _______ transcription.
a) presence, activates
b) absence, activates
c) absence, inactivates
d) presence, inactivates
Q.11.
Tetracycline binding repressor protein when fused to transcription activator protein from herpes simple virus, then it is called as transactivator protein (tTA).
a) True
b) False
Q.12.
Expression of tTA gene is ________ by doxycycline which is _______ analogue.
a) activated, tetracycline
b) inactivated, chloramphenicol
c) activated, chloramphenicol
d) inactivated, tetracycline
Q.13.
Glucorticoid-responsive element is ______ promoter from ______ of mouse mammory tumour virus (MMTV).
a) inducible, short terminal repeat region
b) inducible, long terminal repeat region
c) constitutive, short terminal repeat region
d) constitutive, long terminal repeat region
Q.14.
There are a group of promoters from the genes for metallothioneins. These are a group of proteins rich in ______ residues.
a) cysteine
b) methionine
c) either cysteine or methionine
d) cysteine and methionine both
Q.15.
The amount of expression of DNA inserted into a vector _______ on promoter and ______ on chromosomal location.
a) depends only on, not depends
b) depends, depends also
c) doesn’t depends, but depends
d) neither depends, nor depends
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