Q.1.
on 145 The linking of antibiotic resistance gene with the plasmid Vector became possible with
-
54%
a) DNA ligase
-
0%
b) Exonuclease
-
23%
c) Endo nuclease
-
23%
d) DNA polymerase
Q.2.
on 146 The total number of nitrogenous bases in human genome is estimated to be about
-
50%
a) 35 million
-
8%
b) 3.1 million
-
17%
c) 3.5 million
-
25%
d) 3.5 thousand
Q.3.
on 147 Identify the Plasmid
-
8%
a) Alu I
-
0%
b) Hind III
-
25%
c) EcoRI
-
67%
d) PBR322
Q.4.
on 148 Which of the following is a recombinant protein?
-
25%
a) β galactosidase in E.coli
-
42%
b) Insulin in humans
-
8%
c) Somatotropin in humans
-
25%
d) Interferon in E.coli
Q.5.
on 149 Identify the wrong statements regarding vectors: (i) Vectors include both cloning vectors and expression vectors. (ii) pBR 322, pUC 8 are all examples of cloning vectors. (iii) BAC and YAC are two expression vectors used in Human Genome Project. (iv) Plasmids are vectors which clone only for small fragments of DNA (10 – 50kb). (v) Cloning vectors are all artificial vectors. (vi) Expression vectors are used to make multiple copies of the foreign gene. (vii) Expression vectors are all natural vectors.
-
25%
a) (ii), (iii) and (vi) are wrong
-
38%
b) (iii), (iv) and (vi) are wrong
-
12%
c) (iii) and (vi) are wrong
-
25%
d) (ii) and (vi) are wrong
Q.6.
on 150 Why do bacterial cells contain restriction endonuclease?
-
0%
a) They help identify mutated regions in the chromosome and initiate the excision repair pathway
-
50%
b) By selectively binding to particular nucleotide sequences that may appear in viral DNA, the cell can protect itself from infection
-
10%
c) These help the bacterium open up the DNA in their chromosomes so that transcription is earlier
-
40%
d) They help the bacterium take up foreign DNA from their environment and combine it into their genome
Q.7.
on 151 A principal problem with inserting an unmodified mammalian gene into a bacterial plasmid, and then getting that gene expressed in bacteria, is that
-
30%
a) prokaryotes use a different genetic code from that of eukaryotes
-
20%
b) Bacteria translate polycistronic messages only
-
30%
c) Bacteria cannot remove eukaryotic introns
-
20%
d) Bacterial RNA polymerase cannot make RNA complementary to mammalian DNA
Q.8.
on 152 Repeated amplification is achieved by the use of a thermostable DNA polymerase isolated from:
-
22%
a) Escherichia coli
-
22%
b) Bacillus thuringiensis
-
56%
c) Thermus aquaticus
-
0%
d) Salmonella typhimurium
Q.9.
on 153 The first hormone artificially produced by culturing bacteria is______
-
78%
a) Insulin
-
0%
b) thyroxine
-
22%
c) Testosterone
-
0%
d) Adrenaline
Q.10.
on 154 Plasmids are autonomously replicating mini chromosomes found in......
-
11%
a) Bacteriophage lambda
-
11%
b) Leishmania donovani
-
78%
c) Escherichia coli
-
0%
d) Paramecium caudatum
Q.11.
on 155 Which enzyme is used to break the membrane to release animal DNA?
-
55%
a) Lysozyme
-
9%
b) chitinase
-
9%
c) Cellulose
-
27%
d) All the above
Q.12.
on 156 In AGE, A stands for:
-
0%
a) Accelerated
-
22%
b) Attenuated
-
67%
c) Agarose
-
11%
d) Agar-Agar
Q.13.
on 157 Which of the following is correct for molecular probes?
-
78%
a) Used to detect the complementary sequence in nucleic acid samples
-
11%
b) Used to introduce recombinant DNA into an animal cell
-
0%
c) Used to cut DNA at random positions
-
11%
d) Facilitate the multiplication of DNA
Q.14.
on 158 What will be the effect if pBR322, a cloning vector does not carry ‘Ori’ site?
-
44%
a) Sticky ends will not be produced
-
22%
b) Transformation will not take place
-
11%
c) The cell will transform into a tumour cell
-
22%
d) Replication will not take place
Q.15.
on 159 Restriction endonucleases are used in genetic engineering because -
-
33%
a) They can degrade harmful proteins
-
0%
b) They can join DNA fragments
-
56%
c) They can cut DNA at specific base sequences
-
11%
d) They can cut DNA at variable sites
Q.16.
on 160 EFB stands for........
-
38%
a) European Foundation of Biotechnology
-
12%
b) European Foundation of Biology
-
38%
c) European Foundation of Biotechnology
-
12%
d) European Foundation of Biology
Q.17.
on 161 A genetically engineered microorganism used successfully in bio mediation of oil spill is species of
-
12%
a) Trichoderma
-
25%
b) Xanthomonas
-
12%
c) Bacillus
-
50%
d) Pseudomonas
Q.18.
on 162 Which of the following is not a component of vector pBR 322?
-
25%
a) Chloramphenicol selectable marker
-
25%
b) Replication of proteins
-
12%
c) Origin of replication
-
38%
d) Cloning site for Bam HI enzyme
Q.19.
on 163 Staggered cutting is done by:
-
29%
a) EcoRI
-
57%
b) Hae III
-
14%
c) Hind II
-
0%
d) Sma I
Q.20.
on 164 Select the incorrect statement about biotechnology:
-
43%
a) It means any technique application that uses biological systems, living organisms or derivatives thereof, to make or modify products or processes for specific use
-
14%
b) Biotechnology is an important tool used for the production of food crops, livestock management, human health care, chemical industries and environment management
-
0%
c) It is the study of use of living organisms and the substances produced due to their activities
-
43%
d) Biology of organism in relation to their interdependence
Q.21.
on 165 Two bacteria found to be very useful in genetic engineering experiments are......
-
0%
a) Nitrosomonas and Klebsiella
-
100%
b) Escherichia and Agrobacterium
-
0%
c) Nitrobacter and Azotobacter
-
0%
d) Rhizobium and Diplococcus
Q.22.
on 166 Which primers are used in annealing during amplification of gene ?
-
11%
a) Reverse primers
-
22%
b) Forward primers
-
56%
c) Oligo nucleotide primer
-
11%
d) Internal primers
Q.23.
on 169 Which of the following would you choose as the safest and the most cumbersome selectable marker?
-
25%
a) Ampicillin resistant gene
-
25%
b) Tetracycline resistant gene
-
0%
c) Kanamycin resistant gene
-
50%
d) β galactosidase gene
Q.24.
on 173 The organism whose gene have been artificially altered for desired effect is called as.......
-
12%
a) Genetically mutant organism
-
0%
b) gene transfer
-
50%
c) Genetically modified organism
-
38%
d) Genetically transferred organism
Q.25.
on 174 Name of the drug used in cancer treatment produced by using biotechnology is .....
-
0%
a) HGH
-
0%
b) TSH
-
38%
c) Insulin
-
62%
d) Interferon
Q.26.
on 175 A protein is not expressed properly in a diseased tissue. To find out whether the disease is at the level of translation or post translational modifications, which techniques would you choose?
-
29%
a) Southern blotting and South Western blotting
-
14%
b) Northern blotting and eastern blotting
-
43%
c) Western blotting and eastern blotting
-
14%
d) Southern blotting and northern blotting
Q.27.
on 176 If you discovered a bacterial cell that contained no restriction enzymes, which of the following would you expect to happen?
-
38%
a) The cell would be unable to replicate its DNA
-
0%
b) The cell would create incomplete plasmids
-
50%
c) The cell would be easily infected and lysed by bacteriophages
-
12%
d) The cell would become an obligate parasite
Q.28.
on 177 Ideal host for the amplification of DNA molecules is
-
0%
a) Viruses
-
0%
b) Plants
-
100%
c) Bacteria
-
0%
d) Animals
Q.29.
on 178 Which enzyme is used to break the membrane to release plant DNA ?
-
27%
a) Lysozyme
-
18%
b) Chitinase
-
18%
c) Cellulose
-
36%
d) All the above
Q.30.
on 179 Match the following and choose the correct combination from the option given ...
| Column I | Column II |
| (a) Escherichia coli | 1 - nif gene |
| (b) Rhizobium meliloti | 2 - digestion of hydrocarbons of crude oil |
| (c) Bacillus thuringiensis | 3 - human insulin production |
| (d) Pseudomonas putida | 4 - Biocontrol of fungal disease |
| -- -- | 5 - biodegradable insecticide |
-
62%
a) A = 3, B = 1, C = 5, D = 4
-
0%
b) A = 1, B = 2, C = 3, D = 4,
-
25%
c) A = 2, B = 1, C = 3, D = 4
-
12%
d) A = 4, B = 3, C = 1, D = 2
Q.31.
on 180 Which of the following are true regarding r – DNA technology?
-
0%
a) r – DNA technology is used to obtain large number of copies of specific DNA fragments
-
43%
b) r – DNA technology is used to obtain large quantities of proteins produced by the concerned gene
-
0%
c) r – DNA technology is used to integrate gene of interest into chromosome where its expresses itself
-
57%
d) All of these
Q.32.
on 181 Herbert Boyer and Stanley Cohen genetically modified which bacterium:
-
38%
a) E coli
-
12%
b)Salmonella
-
50%
c) Agrobacterium
-
0%
d) Bacillus
Q.33.
on 182 The nuclease enzyme which begins its attack from Free end of a polynucleotide is
-
43%
a) Exonuclease
-
0%
b) Kinase
-
29%
c) Polymerase
-
29%
d) Endonuclease
Q.34.
on 183 Which one of the following pair is wrongly matched ?
-
50%
a) methanogens - Gobargas
-
12%
b) Yeast - Ethanol
-
0%
c) Streptomycetes - Antibiotic
-
38%
d) Coliforms - vinegar
Q.35.
on 184 Match the columns:
| Column I | Column II |
| a. EcoRI | Chromogenic substrate |
| b. PEG | Restriction endonuclease |
| c. X – Gal | Gene transfer |
| d. Retrovirus | Virus vector |
-
12%
a) a=2, b=4, c=1, d=3
-
50%
b) a=2, b=3, c=1, d=4
-
12%
c) a=1, b=2, c=3, d=4
-
25%
d) a=2, b=1, c=3, d=4
Q.36.
on 185 Introduction of foreign genes into plant or animal cells using micropipettes is
-
0%
a) Electroporation
-
12%
b) Chemical - mediated gene transfer
-
75%
c) Microinjection
-
12%
d) Particle gun
Q.37.
on 186 Particle bombardment technique is also known as .....
-
33%
a) Lipofection
-
67%
b) Electroporation
-
0%
c) Biolistic
-
0%
d) Micro injection
Q.38.
on 187 Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands, what is so special shown in it ? 5' __________ GAATTC ________ 3' 3' __________ CTTAAG ________ 5'
-
14%
a) Replication Completed
-
14%
b) Deletion mutation
-
29%
c) start codon at 5' end
-
43%
d) Palindromic sequence of base pairs
Q.39.
on 188 pBR 322 has restriction sites for:
-
0%
a) Sal I and Cla I
-
17%
b) Hind III and Pvu II
-
33%
c) Pst I and Sal IV
-
50%
d) EcoRI and Bam HI
Q.40.
on 189 Ti Plasmid is useful in
-
0%
a) bringing new genes into animal cells
-
14%
b) bringing new genes into plant cells
-
57%
c) to nearly any sites on a chromosome
-
29%
d) bringing tumour cells into plant cells
Q.41.
on 190 c DNA Probes are copied from messenger RNA molecule with the help of __
-
33%
a) Restriction enzyme
-
33%
b) Reverse transcriptase
-
33%
c) DNA Polymerase
-
0%
d) Adenosine deaminase
Q.42.
on 191 Which of the following statements are true regarding EcoRI? (i) The enzyme name has the first letter from bacterial genes. (ii) The second letter comes from the species. (iii) The forth letter derived from the name of strain. (iv) Roman number indicated the order in which the enzymes were isolated from that strain of bacteria.
-
57%
a) (i), (ii), (iii) and (iv)
-
29%
b) (ii), (iii) and (iv)
-
0%
c) (i) and (ii)
-
14%
d) (iii) and (iv)
Q.43.
on 192 Genes which helps in the growth of transformants are .....
-
17%
a) orgin of replication
-
17%
b) cloning site
-
50%
c) origin of restriction
-
17%
d) selectable marker
Q.44.
on 193 Which of the following statements is not correct regarding EcoRI restriction endonuclease enzyme?
-
14%
a) It is isolated from Escherichia coli RY 13
-
29%
b) Its recognition sequence is
-
29%
c) It produces complementary blunt ends
-
29%
d) EcoRI can produce maximum activity in conditions including low salt concentration, high glycerol concentration, excessive amounts of enzyme present in the reaction, high pH and contamination with certain organic solvents
Q.45.
on 194 In bacteria, genes for antibiotic resistance are usually located in
-
43%
a) Plasmids
-
43%
b) Cytoplasm
-
0%
c) Mitochondria
-
14%
d) Nucleus
Q.46.
on 195 The enzymes that cuts specifically recognition sites in the DNA is known as
-
17%
a) DNA ligase
-
17%
b) DNA Polymerase
-
33%
c) Reverse transcriptase
-
33%
d) Restriction endonuclease
Q.47.
on 196 Plasmids are the suitable vectors for genetic cloning as.....
-
0%
a) They are indispensable
-
50%
b) They are self-replicating units
-
25%
c) They are essential for bacterial reproduction
-
25%
d) None of the above
Q.48.
on 197 Ti Plasmid naturally occurs in
-
67%
a) Agro bacterium
-
0%
b) Corynebacterium
-
33%
c) Staphylococcus
-
0%
d) Vibrio
Q.49.
on 198 The sequence of DNA that reads the same backward and forward across the double strand is........
-
14%
a) Recipient sequence
-
71%
b) palindromic sequence
-
14%
c) Replicate sequence
-
0%
d) origin sequence
Q.50.
on 199 What is C - DNA ?
-
0%
a) circular DNA
-
50%
b) Cloned DNA
-
0%
c) DNA produced from reverse transcription of RNA
-
50%
d) Cytoplasmic DNA