For transformation with recombinant DNA, the bacterial cells must first be made ‘competent’ which means: {submitted by Adhithyan js}

Two enzymes responsible for restricting the growth of bacteriophages in Escherichia coli were isolated inOne was methylase and the other was restriction endonuclease. What was the significance of methylase? {submitted by Adhithyan js}

Select the correct option which shows the most appropriate temperature of three different steps of PCR mechanism:{submitted by Adhithyan js}

Sr	Denaturation	primer annealing	primer extension
A	40 - 60°C	72°C	90°C
B	60 – 80°C	96 ºC	40 – 60°C
C	94 – 96°C	40 – 60°C	72ºC
D	72°C	60 – 80°C	40 – 60°C

5’-GAATTC-3’. Arrange the correct 3’-5’ strand. {submitted by Adhithyan js}

Which of the following is not true about pBR322 vector? {submitted by Adhithyan js}

Which of the following is used as a vector for introducing a DNA fragment into human lymphocytes? {submitted by Adhithyan js}

Identify the wrong statement: {submitted by Adhithyan js}

Identify the parts labelled 1-5 in the given diagram of simple stirred tank bioreactor: {submitted by Adhithyan js}

What type of bond is formed by the sticky ends with their complementary counterparts: {submitted by Adhithyan js}

Which is incorrect? {submitted by Adhithyan js}

Match the following: {submitted by Adhithyan js}

Column I	Column II
Hae III	a. Arthrobacter luteus
2.Pst I	b. Proteus vulgaris
3.Alu I	c. Haemophilus aegyptius
4.Pvu I	d. Providencin staurtii

If a eukaryotic gene is incorrectly inserted into a plasmid vector the recipient bacterium may fail to express the gene because it cannot recognise the eukaryotic: {submitted by Adhithyan js}

In PUC 8 foreign gene is inserted into: {submitted by Adhithyan js}

The polymerase chain reaction generates fragments of distinct size even when an intact chromosome is used as template. What determines the boundaries of the amplified fragment? {submitted by Adhithyan js} (

First instance of artificial recombinant DNA molecule was constructed by: {submitted by Adhithyan js}

Select the correctly matched pair: (i) Coenorhabditis elegans – A free living pathogenic nematode (ii) Expressed sequence tags – Identifying all the genes that are expressed in DNA. (iii) Molecular scissors- Restriction enzyme. (iv) Microinjection – Separation of DNA fragments. (v) Kanamycin resistant gene – Selectable marker for Ecoli.{submitted by Adhithyan js}

Which of the following is true about exonucleases? {submitted by Adhithyan js}

Match the following columns I and II.{submitted by Adhithyan js}

Column I	Column II
DNA ligase	a. Cuts DNA approximately 25 nitrogen bases away from the recognition sequence
Type II restriction enzyme	b. Cuts DNA 1000 nitrogen bases away from the recognition sequence
Type I restriction enzyme	c. Joins the ends of DNA
Type III restriction enzyme	d. Cuts DNA at the recognition sites

An ideal vector should have selectable markers for transformation. Usually these are antibiotic resistant genes. The other genes which can be used are: {submitted by Adhithyan js}

Telomerase enzymes were discovered by: {submitted by Adhithyan js}

Replica plating is used to identify recombinant Ecoli when which vector is used: {submitted by Adhithyan js}

Which of the following statement is wrong: {submitted by Adhithyan js}

Variability seen in the human genome at 1.4 million locations is called: {submitted by Adhithyan js}

Which of the following contains the key tools for recombinant DNA technology? (i) Restriction endonuclease, Ligase, Vectors. (ii) Ligase, Host organisms, polymerase enzymes. (iii) Vectors, Taq polymerase, primers. (iv) Restriction exonucleases, Ligase, primers, bioreactors.{submitted by Adhithyan js}

Which of the following is required to perform Polymerase chain reaction: {submitted by Adhithyan js}

The number of cloning sites(restriction sites) in pBR322: {submitted by Adhithyan js}

If any protein encoding gene is expressed in an heterologous host called: {submitted by Adhithyan js}

Match column I with column II with respect to the nomenclature of restriction enzyme EcoRI and select the correct answer from the codes given below:{submitted by Adhithyan js}

Column I	Column II
a. E	i. Ist in order of identification
b.co	(ii) name of genus
c. R	(iii) name of species
d. I	(iv) name of strain.

Which one is correct in gene isolation: {submitted by Adhithyan js}

A piece of DNA introduces into an alien organism’s cytoplasm cannot multiply. But it can multiply when incorporated into DNA or chromosome of the alien cell. This is due to: {submitted by Adhithyan js}

Consider the following statements and select the correct option: (a) The first restriction endonuclease isolated was Hind III. (b) The most commonly recognised sequences of DNA are 4,5 or 6 base pairs long. (c) Restriction endonuclease is called molecular scissors. (d) Restriction enzymes remove the phosphate group from the 5’ end of DNA. (e) The action of restriction enzymes generates both staggered and blunt ends.{submitted by Adhithyan js}

Number of strains from which restriction enzymes have been isolated; {submitted by Adhithyan js}

Match the following: {submitted by Adhithyan js}

E.coRI	(a) Bacillus amyloliquefaciens.
Bam HI	(b) Haemophilus influenzae.
Hind III	(c) Eschericha coli.
pBR322	(d) Artificial plasmid.

Carefully check the following steps. Identify the process and label the steps: {submitted by Adhithyan js}

Basis of DNA fingerprinting is: {submitted by Adhithyan js}

Which of the following is not required for PCR? {submitted by Adhithyan js}

Setting up a bioreactor with sterile medium and culturing the biological agent is: {submitted by Adhithyan js}

Restriction endonuclease functions by inspecting:{submitted by Adhithyan js}

Read the following statements carefully and identify the plasmid; (i) This is constructed from the plasmids of Ecoli, pBR 318 and pBR (ii) It contains origin of replication (ori) that was derived from a plasmid related to naturally occurring plasmid Col E. (iii) It also possesses genes conferring resistance to antibiotics-Ampicillin (ampr) and tetracycline (tetr), and unique recognition sequence for 40 restriction endonuclease. (iv) It is 4,363 bp in size.{submitted by Adhithyan js}

Cosmids are hybrid vectors constructed by using parts of lambda chromosome and plasmid DNA. The cos sequence of phage lambda in cosmid is necessary for: {submitted by Adhithyan js}

Palaeontologists unearthed a human skull during excavation. A small fragment of the scalp tissue was still attached to it. Only a little DNA could be extracted from it. If the genes of the ancient man need to be analysed the best way of getting sufficient amount of DNA from this extract is: {submitted by Adhithyan js}

You are attempting to introduce a gene that imparts larval moth resistance to bean plants. Which of the following vectors are more likely to be used? {submitted by Adhithyan js}

Which of the following statement is not true regarding downstream processing? i) This includes purification and separation. (ii) The product has to be formulated with suitable preservatives. (iii) Such formulations have to undergo through clinical trials in case of drugs. (iv) The downstream processing and quality control does not vary from product to product. (v) Downstream processing is the name given to the stage after fermentation when desired products are recovered and purified. {submitted by Adhithyan js}

The segments of DNA produced has restriction sites I and II which creates restriction fragments a, b and c. Which one of the following gel produced by electrophoresis would represent the separation and identity of these fragments? {submitted by Adhithyan js}

A plasmid has two antibiotic resistant genes, one for Ampicillin and one for tetracycline. It is treated with a restriction enzyme that cut in the middle of the Ampicillin gene. DNA fragment containing a human globin genes were cut with the same enzyme. The plasmids and fragments are mixed, treated with ligase and used to transform bacterial cell. Clones that have taken up recombinant DNA are the ones that: {submitted by Adhithyan js}

The given diagram shows Ecoli cloning vector pBR 322 in which numbers represent: {submitted by Adhithyan js}

Bacteria protect themselves from viruses by fragmenting viral DNA with .. [ Odisha 2008]

Complementary, synthetic and random DNAs are used as ...[ CET Chd 2011]

Introduction of foreign gene for improving genotype is ...[ AIIMS 2002]

Bt toxin is not toxic to human beings as ... [ AMU 2011]