ion 97 During which of the following techniques host cells are exposed to pulse of high voltage current ?
57%
a) Electroporation
0%
b) Particle Bombardments
43%
c) Micro injection
0%
d) lipofection
Q.3.
ion 98 TATAAT sequence near the RNA start point of prokaryotic promoter is......
14%
a) NICKS
0%
b) DNA marker
57%
c) palindrome
29%
d) pribnow box
Q.4.
ion 99 Assertion - Extraction and purification of enzymes is laborious and expensive Reason - protein engineering can be used to produce enzymes at large scale
33%
a) Assertion is correct, Reason is explanation of Assertion
33%
b) Assertion is correct, Reason is correct but it is not explanation of Assertion
33%
c) Assertion is correct, Reason is false
0%
d) Assertion is wrong, Reason is wrong
Q.5.
on 100 Production of a human protein in bacteria genetic engineering is possible because
17%
a) Bacterial cell can carry out the RNA splicing reactions
17%
b) The human chromosome can replicate in bacterial cell
33%
c) The mechanism of gene regulation is identical in human and bacteria
33%
d) The genetic code is universal
Q.6.
on 101 Variable number of tender repeats (VTNR) in the DNA molecule are highly useful in
0%
a) Monoclonal antibody production
83%
b) DNA finger printing
17%
c) Recombinant DNA technology
0%
d) stem cell culture
Q.7.
on 102 A technology which has found immense use in solving cases of disputed parentage is
17%
a) DNA finger printing
33%
b) Polymerase chain reaction
50%
c) Recombinant DNA technology
0%
d) Monoclonal antibody production
Q.8.
on 103 Why is a marker gene useful?
0%
a) It shows you where the gene being added has been taken up
25%
b) Its a way of labelling which gene you want to modify
50%
c) It sticks with the striker gene
25%
d) It marks the position of r-DNA
Q.9.
on 104 Paul Berg's gene splicing experiment created the first r DNA molecule which was a:
67%
a) T4 phage fragment incorporated into SV40 vector
0%
b) 位 phage fragment incorporated into SV40 vector
0%
c) T4 phage fragment incorporated into PSC101 vector
33%
d) 位 phage fragment incorporated into PSC 101 vector
Q.10.
on 105 Electroporation is
0%
a) the process of separating charged molecules through a gel maintained in an electric field
50%
b) the process of combining foreign DNA to an electrically charged vector molecule
25%
c) the application of high voltage pulses
25%
d) the process of multiplication of the cells
Q.11.
on 106 How does nucleic acid probe hybridize with a specific target sequence?
33%
a) DNA binding regions of the probe form motifs that can align along the major groove and read the appropriate sequences
33%
b) Probes will cut and integrate into specific regions of the DNA being analyzed, thereby making the desired location
33%
c) Base – pairing between single stranded probes and denatured target DNA allows them to anneal
0%
d) Reverse transcriptase amplifies the probe, which can then attract the target sequence from the DNA under analysis
Q.12.
on 107 A genomic library is:
0%
a) a database where the sequence of an organism's genome is stored
75%
b) a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
25%
c) a book that describes how to isolate DNA from a particular organism
0%
d) a place where the information of the genetic organization of organisms are kept
Q.13.
on 108 Bacteria containing recombinant plasmids are often identified by which process?
0%
a) examining the cells with an electron microscope
40%
b) using radioactive tracers to locate the plasmids
20%
c) exposing the bacteria to an antibiotic that kills cells lacking the resistant plasmid
40%
d) removing the DNA of all cells in a culture to see which cells have plasmids
Q.14.
on 109 Exonucleases cleaving nucleotides one at a time from the end of polynucleotide chain are.
33%
a) Specific for 5’ end of RNA strand
0%
b) specific for 3’ end of RNA strand
67%
c) specific for both 5’ and 3’ ends of nucleotide strand
0%
d) Non- specific for 5’ and 3’ ends of nucleotide
Q.15.
on 110 SNP’s (Single nucleotide polymorphism) refers:
25%
a) Location of RNA where a single base pair differs
25%
b) Location of RNA where a single base differs
50%
c) Location on the genome where a single base pair of DNA differs
0%
d) Location on the genome where a single base of DNA differs
Q.16.
on 111 Which of the Following is a genetic vector?
67%
a) Plasmid
0%
b) Phage
33%
c) Cosmid
0%
d) All of these
Q.17.
on 112 When the number of genes increases in response to some signal the effect is called.....
0%
a) gene dosage
50%
b) Gene pool
25%
c) gene amplification
25%
d) gene frequency
Q.18.
on 113 Ti Plasmid is a cloning vector which works with
33%
a) All the plants
33%
b) Dicots only
33%
c) Monocots only
0%
d) Thallophytes only
Q.19.
on 114 Identify the process in PCR which takes place at low temperature:
50%
a) Denaturation
25%
b) Annealing
0%
c) Synthesis
25%
d) Extension
Q.20.
on 115 For transformation, micro particles coated with DNA are bombarded with gene gun made up of.
33%
a) Platinum or Zinc
33%
b) Silicon or Platinum
33%
c) Gold or tungsten
0%
d) Silver or Platinum
Q.21.
on 116 What is the function of Restriction endonuclease?
0%
a) Restricts the synthesis of DNA inside the nucleus
25%
b) Synthesizes DNA
0%
c) Cuts DNA molecule randomly
75%
d) cuts DNA molecule at specific sites
Q.22.
on 117 Widely used tool in genetic engineering of crop plants is ____
0%
a) Protoplast fusion
0%
b) Transposon
67%
c) Micro injection
33%
d) Agrobacterium mediation
Q.23.
on 118 A distinct advantage of continuous culture over batch culture is:
33%
a) Higher productivity
0%
b) Versatility
67%
c) Can be used for different reactions every day
0%
d) Continuous inflow of nutrients
Q.24.
on 119 Which is true? a) Biowar is the use of biological weapons against humans or their crops and animals. b) Bioethics is the unauthorized use of bio resources & traditional knowledge related to bio resources for commercial benefits. c) Biopatent is exploitation of bio resources of other nations without proper authorization.
50%
a) a & c
50%
b) a
0%
c) a & b
0%
d) b & c
Q.25.
on 120 If gene of interest is cloned at Pst I site of pBR 322 and resultant plasmid is transformed into E coli. Recombinant E coli will exhibit susceptibility to:
0%
a) Tetracycline
0%
b) Ampicillin
40%
c) Chloramphenicol
60%
d) Both A and B
Q.26.
on 121 Which of the following statements is not correct regarding PCR?
0%
a) The number of molecules increases exponentially like an explosive chain reaction, is also called chain reaction
50%
b) DNA must be cooled to 40ºC to allow the primers to anneal to their complementary sequences on the separated DNA strand
25%
c) Denaturation is the first step of PCR reaction and involves the breaking of hydrogen bonds
25%
d) DNA double helix would be separated by the enzyme helicase in PCR
Q.27.
on 122 Read the following statements and select the correct ones: (i) Electrophoresis is a technique used for the separation of substances based on their size and charge. (ii) Plasmids are extra – chromosomal, self-replicating, usually circular, double stranded DNA molecules found naturally in many bacteria and also in some yeast. (iii) It is not advisable to use an exonucleases enzyme while producing a recombinant DNA molecule. (iv) In EcoRI, the Roman numeral I indicates that it was the first enzyme isolated from Escherichia coli RY 13.
0%
a) (i) and (ii)
25%
b) (iii) and (iv)
25%
c) (i), (ii) and (iv)
50%
d) (i), (ii), (iii) and (iv)
Q.28.
on 123 Leaf disc transformation method is used in transferring genes into:
0%
a) Wheat
0%
b) Rice
33%
c) Barely
67%
d) Tobacco
Q.29.
on 124 Type I restriction enzyme:
75%
a) Recognize the specific sequence and cut DNA at the specific sites
25%
b) Do not recognize or cut the specific sequences
0%
c) Recognize specific sequences but remove nonspecific sequences
0%
d) None of the above
Q.30.
on 125 Extra chromosomal small circular double stranded DNA molecule in a bacterial cell is stranded DNA molecule in bacterial cell is
0%
a) Plastid
75%
b) Plasmid
25%
c) Mitochondrion
0%
d) Chloroplast
Q.31.
on 126 The sticky ends of Fragmented DNA molecules are made up of
25%
a) Calcium salts
25%
b) endo nuclease
25%
c) unpaired bases
25%
d) methyl groups
Q.32.
on 127 Improvement of genotype of an organism by addition of some foreign gene is......
40%
a) Genetic diversity
0%
b) gene handing
20%
c) Tissue culture
40%
d) genetic engineering
Q.33.
on 128 What is temperature required for annealing of DNA molecule?
25%
a) 50-65° C
75%
b) 30-35° C
0%
c) 40-45° C
0%
d) 20-25° C
Q.34.
on 129 Enzymes used in PCR is ....
0%
b) gyrase
0%
c) transcriptase
100%
a) Taq polymerase
0%
d) hexokinase
Q.35.
on 130 Assertion - Restriction enzymes of different organisms that recognize the identical sequences are called isoschizomers. Reason - They are present only in eukaryotes
50%
a) Assertion is correct, Reason is explanation of Assertion
25%
b) Assertion is correct, Reason is correct but it is not explanation of Assertion
25%
c) Assertion is correct, Reason is false
0%
d) Assertion is wrong, Reason correct
Q.36.
on 131 PCR and Restriction fragment length Polymorphism are the methods for.
50%
a) genetic transformation
25%
b) DNA Sequencing
25%
c) DNA finger printing
0%
d) Study of enzymes
Q.37.
on 168 Which of the following pair is correctly matched ?
0%
a) Central dogma - codon
0%
b) Okazaki fragments - splicing
0%
c) RNA Polymerase - RNA Primer
100%
d) Restriction enzymes - genetic engineering
Q.38.
on 132 The basis of DNA finger printing is
0%
a) The double helix
0%
b) Errors in base sequence
33%
c) Poly morphism in sequence
67%
d) DNA replication
Q.39.
on 133 First Biochemical to be produced commercially by microbial cloning and genetic engineering is ___
25%
a) Interferon
50%
b) penicillin
25%
c) Human insulin
0%
d) Fertility factors
Q.40.
on 134 Which one of the following is a wrong matching of a microbe and its industrial product while the remaining three are correct
0%
a) Clostridium acetobutylicum - lactic acid
33%
b) Aspergillus niger - citric acid
67%
c) yeast - statins
0%
d) Acetobacter aceti - acetic acid
Q.41.
on 135 In PCR, which of the following is used to produce multiple copies of DNA?
0%
a) Agrobacterium tumefaciens
25%
b) Escherichia coli
50%
c) Thermus aquaticus
25%
d) Bacillus thuringiensis
Q.42.
on 136 The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of
75%
a) genetic engineering
25%
b) population genetics
0%
c) microbiology
0%
d) protein engineering
Q.43.
on 137 What type of vectors was used for introducing nematode specific genes in the host plant?
25%
a) Plasmid vectors
50%
b) Retroviruses
25%
c) Phasmid vectors
0%
d) Agrobacterium vectors
Q.44.
on 138 Read the given statements and select the correct option: Statement 1: In Insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome. Statement 2: Presence of insert results into Insertional inactivation of β galactosidase enzyme and the colonies do not produce any colour.
25%
a) Both statements -1 and statement - 2 are true and statement - 2 is the correct explanation of statement - 1
25%
b) Both statements -1 and statement - 2 are true but statement - 2 is not the correct explanation of statement - 1
50%
c) Statement -1 is true and statement - 2 is false
0%
d) Statement -1 is false and statement - 2 is true
Q.45.
on 139 Transgenic organisms means:
0%
a) An organism with all desirable genes
33%
b) An organism with a number of mutated characters
67%
c) An organism with foreign gene produced by genetic engineering
0%
d) An organism with a particular mutated gene
Q.46.
on 140 The Ti plasmid of Agrobacterium contains gene for the production of an amino acid analogue called:
33%
a) Auxin
0%
b) Cytokinin
33%
c) Opines
33%
d) Agarose
Q.47.
on 141 How many of the following statements are true: (i) Stirred tank bioreactor is well stirred and tested design for large scale production of enzymes, proteins, etc. (ii) pBR 322 is an artificially reconstructed plasmid vector from the plasmid of E coli. (iii) In gel electrophoresis, DNA fragments are placed over the anode end. (iv) Prokaryotic DNA has normally a single origin of replication. (v) A bacterium which is modified through a foreign gene is called recombinant.
25%
a) 1
75%
b) 2
0%
c) 3
0%
d) 4
Q.48.
on 142 Many copies of a DNA molecule in a test tube are procured by
40%
a) Polymerase chain reaction (PCR)
20%
b) Molecular chain reaction (MCR)
40%
c) Ephemeral chain reaction (ECR)
0%
d) All of these
Q.49.
on 143 Which of the following is used in genetic engineering?
50%
a) Restriction endonuclease
25%
b) Mycobacterium
25%
c) Entamoeba
0%
d) Pepsin
Q.50.
on 144 How many restriction enzymes are known to be isolated?
25%
a) More than 800
25%
b) more than 700
50%
c) More than 600
0%
d) more than 900
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