Only one cycle of directed evolution cycle is sufficient to obtain the desired protein molecule.
a) True
b) False
Q.2.
The starting point of both rational and irrational protein design is a wild-type protein.
a) False
b) True
Q.3.
Which step is performed after the mutation of genes in directed evolution?
a) X-ray crystallography
b) Protein assay
c) Protein screening
d) Expression of mutant genes
Q.4.
Which of the following is not a method of directed evolution?
a) Phage display
b) DNA shuffling
c) Error-prone PCR
d) Natural selection
Q.5.
Who pioneered phage-display technology?
a) Kary Mullis
b) Charles Chamberland
c) Robert brown
d) George Smith
Q.6.
In the case of monovalent display, the valency of display depends on the ratio of phage containing phagemid vector and helper phage at the time of infection.
a) False
b) True
Q.7.
Which of the following bacteriophage is used in phage-display technology?
a) T4
b) λ
c) T2
d) M13
Q.8.
Mis a filamentous phage and it contains a circular single-stranded DNA genome.
a) False
b) True
Q.9.
Which of the following is the major coat protein of M13?
a) gp VI
b) gp V
c) gp IV
d) gp VIII
Q.10.
Which of the following is not true for error-prone PCR?
a) It uses Taq polymerase which lacks proofreading ability
b) Occurs under imperfect PCR conditions
c) Involves annealing incompatible base-pairs to each other
d) It is not a mutagenesis method
Q.11.
Which of the following proteins are involved in initiating phage assembly and maintaining the stability of the viral particle?
a) gp III and gp VIII
b) gp VIII and gp II
c) gp VI and gp IV
d) gp VII and gp IX
Q.12.
In the case of a monovalent display, the protein fusion can be constructed in a phagemid vector that carries a copy of the viral capsid gene.
a) False
b) True
Q.13.
Which of the following is used to create a large library of mutants for a given protein gene?
a) Protein assay
b) Phage display
c) X-ray crystallography
d) Error-prone PCR
Q.14.
In the case of polyvalent-display, all gp III are conjugated with peptide and this results in occasional loss of infectivity.
a) False
b) True
Q.15.
In the case of a polyvalent display, the proteins can be cloned directly into a viral vector as a fusion to a capsid protein.
a) False
b) True
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