Annealing is the second step in PCR. This is when:
DNA is copied
DNA strands separate
Primers bind to DNA
The PCR is finished
Q.2.
What is the function of a primer?
To identify the particular region of DNA to be copied by PCR.
To copy DNA.
To create DNA nucleotides.
To maintain the temperature of the PCR reaction.
Q.3.
With regards to DNA replication along the template strand, what event takes place first?
DNA polymerase begins adding RNA nucleotides
DNA polymerase begins adding DNA nucleotides
DNA ligase joins the Okazaki fragments
primase adds an RNA primer
Q.4.
All of the following are thermostable polymerases except
Taq Polymerase
Vent Polymerase
DNA Polymerase III
pfu Polymerase
Q.5.
You decide to use PCR to determine if you have Cystic Fibrosis, a genetic disorder. You then decide to also check if you are at risk for developing Alzheimers, another genetic disorder.What PCR "ingredient" would be different in these two tests?
Template DNA
Nucleotides
Taq DNA polymerase
Primers
Q.6.
Thermus aquaticus is the source of
Primase enzyme
Taq Polymerase
Vent Polymerase
Both 2 and 3
Q.7.
Which of the following statements about DNA replication is TRUE?
the leading strand is replicated continuously, while the lagging strand is replicated discontinuously
the leading strand is replicated discontinuously, while the lagging strand is replicated continuously
both the leading and lagging strands are replicated continuously
both the leading and lagging strands are replicated discontinuously
Q.8.
During PCR, what is the purpose of increasing temperature to°\degree° C?
For primers to anneal to DNA template strands
To separate DNA strands
To denature Taq polymerase
For Taq polymerase to form new copies of DNA
Q.9.
Which of the following is true regarding PCR
Denaturation involves heating at 90-98 degree C
Annealing involves binding of primer between 40-60 degree C
Primer extension occurs at 72 degree C
All of these
Q.10.
The PCR technique was developed by
Karry Mullis
Kohler
Milstein
Altman
Q.11.
PCR is a
DNA Sequencing technique
DNA Amplification technique
DNA Degradation technique
All of these
Q.12.
The basic requirements of PCR reaction include
DNA segment to be amplified
Two oligonucleotide primers
a heat stable DNA polymerase
all of these
Q.13.
The first step in PCR is
Primer annealing
Denaturation
Primer extension
None of these
Q.14.
Denaturation involves
heating between 40-60 C
heating between 90-98 C
heating between 70-72 C
None of these
Q.15.
Which of the following statements are true regarding PCR
Automated PCR machines are called thermal cyclers
A thermostable DNA Polymerase is required
Billion copies of desired DNA can be synthesized from microgram quantities of DNA
All of these
Q.16.
Reverse transcription PCR uses
mRNA as a template to form
RNA as a template to form a DNA
DNA as a template to form ssDNA
all of these
Q.17.
If two double-stranded DNA molecules are used at the beginning of a polymerase chain reaction (PCR) process, how many double-stranded DNA molecules can be obtained after two cycles?
2
4
8
32
Q.18.
How is Fast PCR able to run at such a shorter time frame
Lower the temperature of Denaturation
raises the temperature for annealing so that it and extension can happen almost simultaneously
Shortens the time frame of denatureation
all of the answers are correct
Q.19.
What is the function of DNA Polymerase?
attach primers to target sequence
separate the DNA
attach nucleotides to the target sequence
separate the fragments according to size
Q.20.
If the primer sequence is TCG, what section of DNA will it anneal?
AGC
CAT
GAC
ATC
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