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Quiz 3
Solution
Q.1.
What happens during the Anneal step of PCR?
Primers are created.
The primers attach to the target DNA region.
Primers copy the new DNA strand.
Primers sequence DNA
Q.2.
What is the purpose of the Extension step of PCR?
To create the primers.
To extend the time it takes to produce DNA.
To allow polymerase to create the complementary strands of DNA.
To allow substrates to copy DNA sequences.
Q.3.
How many cycles of PCR are normally used.
20-35
6
45
100
Q.4.
What happens in the Denature step of PCR?
The DNA nucleotides are broken apart.
The base-pairing rules for DNA are reversed.
The double-stranded DNA is separated into two single strands of DNA.
The DNA is returned to its natural setting.
Q.5.
PCR happens in 3 steps (denaturing, annealing, extending) or 1 "cycle". How many times are cycles typically repeated?
1
10
30
Over 1 billion
Q.6.
What are the base-pairing rules in DNA?
A-C, G-T
A-U, G-C
A-T, G-C
A-C, G-U
Q.7.
How many cycles of PCR does it take to produce a double-stranded DNA fragment of the desired length?
1
2
3
4
Q.8.
Why does PCR require a specific type of polymerase?
Taq polymerase can withstand the range of temperatures in PCR without denaturing.
Taq polymerase is the most inexpensive type of polymerase.
Taq polymerase is the most common type of polymerase.
A specific type of polymerase is unnecessary.
Q.9.
At what temperature does the Extension step of PCR occur?
94-96°C
50-65°C
72°C
Any temperature
Q.10.
What does PCR stand for?
Polymerase Chronic Reagent
Principle Chorionic Reliability
Polymerase Chain Reaction
Probably Cannot React
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