Explanation: Lectins are antibodies; that is they play the role of ligand in protein-ligand interactions. Lectins are used to identify glycoproteins.

Explanation: Northern blotting technique is a variant of Southern blotting technique and is used exclusively for the analysis of RNA.

Explanation: In Northern blotting, RNA bands are blot-transferred from the gel onto a chemically reactive paper, where they are bound covalently.

Explanation: The reactive paper is prepared by diazotization of aminobenzyloxymethyl paper, which itself can be prepared from Whatman 540 paper.

Explanation: Once covalently bound, the RNA is available for hybridization. Bands can be located by autoradiography.

Explanation: It was found in 1980 by Thomas that RNA bands can be blotted onto nitrocellulose membranes under appropriate conditions.

Explanation: Suitable nylon membranes have been developed for efficient binding of RNA, because of the convenience of these membranes; the need for DBM paper has been superseded.

Explanation: The term Western blotting refers to a procedure which does not directly involve nucleic acids, but which is of importance in gene manipulation.

Explanation: Western blotting techniques are used to transfer the protein molecules from acrylamide gels to membranes for further procedures.

Explanation: The technique was developed by Brunette in 1981. Western blotting techniques are used to transfer the protein molecules from acrylamide gels to membranes for further procedures.

Explanation: Western blotting involves a transfer of electrophoresed protein bands from a polyacrylamide gel on to a nitrocellulose or nylon membrane.

Explanation: In the western blotting technique, nitrocellulose or nylon membranes are used which aid in strong binding of the sample.

Explanation: The bound proteins are available for analysis by a variety of specific protein-ligand interactions. Most commonly antibodies are used.

Explanation: Ligands are used to facilitate protein-ligand interactions in the blotting technique. The most common ligands used are antibodies.

Explanation: Lectins are basically ligands and help in the detection of a major class of proteins called the glycoproteins. They promote protein-ligand interaction.